Simultaneous Determination Of Pheniramine Maleate, Pantoprazole, Pohsphated Riboflavin And Adenosine By Hilic And Validated Method For Quanitification Of Adenosine.
Abstract
A Hydrophilic interaction liquid chromatography (HILIC) method was developed for seperation of mixture of compounds Pheniramine maleate, Pantoprazole, Pohsphated Riboflavin and Adenosine by HILIC and validated method for quanitification of Adenosine. Agilent 1260 infinity HPLC system equipped with a photodiode array detector was utilised. A segmented gradient mode of speration was applied at a constant temperature of 35°C initial mobile phase composition was 3% of 5mM ammonium acetate buffer at pH 3.8 and 97% Acetonitrile. Then the % of buffer was increased to 10% in 5 minutes and then to 55% in 15 minutes. 250 nm was used as detector Wavelenght. The developed method was also studied to test its stability for degraded sample of Adenosine under Acid, Base, Peroxide, Heat and combination. Validation study to test the method’s specificity, accuracy, linearity, precision for analysis of Adenosine was also perfomed.
Simultaneous Determination Of Pheniramine Maleate, Pantoprazole, Pohsphated Riboflavin And Adenosine By Hilic And Validated Method For Quanitification Of Adenosine.
A Hydrophilic interaction liquid chromatography (HILIC) method was developed for seperation of mixture of compounds Pheniramine maleate, Pantoprazole, Pohsphated Riboflavin and Adenosine by HILIC and validated method for quanitification of Adenosine. Agilent 1260 infinity HPLC system equipped with a photodiode array detector was utilised. A segmented gradient mode of speration was applied at a constant temperature of 35°C initial mobile phase composition was 3% of 5mM ammonium acetate buffer at pH 3.8 and 97% Acetonitrile. Then the % of buffer was increased to 10% in 5 minutes and then to 55% in 15 minutes. 250 nm was used as detector Wavelenght. The developed method was also studied to test its stability for degraded sample of Adenosine under Acid, Base, Peroxide, Heat and combination. Validation study to test the method’s specificity, accuracy, linearity, precision for analysis of Adenosine was also perfomed.