Interaction of Anxiety and TCB-2: A Serontengeic Agonist
Location
FA 202
Department
Psychology
Abstract
Antidepressants have been found to cause dysregulation of the autonomic nervous system. The purpose of this research is to show the activation of the 5HT2A serotonergic neuroreceptor agonist as a substitute for everyday SSRIs to depression. Adult male and female zebrafish will be randomly selected and divided into two groups, control (n=10) and experimental (n=10). In both groups, anxiety will be induced by housing the subjects individually for the duration of the experiment, but the experimental group will be dosed with TCB-2 (5HT2A agonist, 100 microM). Subjects will receive 12 doses of TCB-2 over the time period of 6 days with behavior measures taken every other day. To assess anxiety swim behavior will be recorded in a novel tank (6in x 2in x 6in). Anxiety level will be measured using 3 methods: average velocity (pixels/ms), bottom dwelling (sec), and latency (sec) to the top of the tank. Comparisons will be made between control and experimental groups. Results are expected to show a decrease in average velocity, a decrease in bottom dwelling, and a decrease in latency to the top after treatment with TCB-2. The activation of the 5HT2A serotonergic neuroreceptor has potential to be utilized as a method to decrease anxiety symptoms without some of the potential side effects that are seen with typical SSRIs that are used to treat depression or anxiety.
Faculty Sponsor
Shannon Saszik, Northeastern Illinois University
Interaction of Anxiety and TCB-2: A Serontengeic Agonist
FA 202
Antidepressants have been found to cause dysregulation of the autonomic nervous system. The purpose of this research is to show the activation of the 5HT2A serotonergic neuroreceptor agonist as a substitute for everyday SSRIs to depression. Adult male and female zebrafish will be randomly selected and divided into two groups, control (n=10) and experimental (n=10). In both groups, anxiety will be induced by housing the subjects individually for the duration of the experiment, but the experimental group will be dosed with TCB-2 (5HT2A agonist, 100 microM). Subjects will receive 12 doses of TCB-2 over the time period of 6 days with behavior measures taken every other day. To assess anxiety swim behavior will be recorded in a novel tank (6in x 2in x 6in). Anxiety level will be measured using 3 methods: average velocity (pixels/ms), bottom dwelling (sec), and latency (sec) to the top of the tank. Comparisons will be made between control and experimental groups. Results are expected to show a decrease in average velocity, a decrease in bottom dwelling, and a decrease in latency to the top after treatment with TCB-2. The activation of the 5HT2A serotonergic neuroreceptor has potential to be utilized as a method to decrease anxiety symptoms without some of the potential side effects that are seen with typical SSRIs that are used to treat depression or anxiety.