Chemical Characterization and Behavioral Study of Water Solubilized CBD Extract on Zebrafish
Location
Poster #21
Department
Chemistry
Abstract
CBD products have taken a hold in the commercial space, both in unregulated markets and in regulated dispensary markets. They are frequently marketed as a method to reduce anxiety and pain, or to promote relaxation and sleep. Additionally, certain CBD-containing products have been approved for use as medicine, such as for treatment of childhood epilepsy. Numerous studies have been performed that demonstrate this cannabinoid’s effects on vertebrate health and behavior, from zebrafish to mice to humans. In this study, we wish to observe the behavioral effects of hemp-derived cannabinoids on zebrafish, with an emphasis on anxious behaviors. Because cannabinoids are largely non-polar substances that demonstrate higher affinity for non-polar solvents than with water, we plan to extract cannabinoids from hemp with hexane, then into DMSO for dosing the zebrafish subjects. If we can demonstrate an effect, we hope to expand into comparisons with extracts containing different proportions of cannabinoids and full-spectrum extracts of hemp flower that contain terpenes and flavonoids in addition to cannabinoids.
Faculty Sponsor
Shannon Saszik
Faculty Sponsor
Charles Abrams
Chemical Characterization and Behavioral Study of Water Solubilized CBD Extract on Zebrafish
Poster #21
CBD products have taken a hold in the commercial space, both in unregulated markets and in regulated dispensary markets. They are frequently marketed as a method to reduce anxiety and pain, or to promote relaxation and sleep. Additionally, certain CBD-containing products have been approved for use as medicine, such as for treatment of childhood epilepsy. Numerous studies have been performed that demonstrate this cannabinoid’s effects on vertebrate health and behavior, from zebrafish to mice to humans. In this study, we wish to observe the behavioral effects of hemp-derived cannabinoids on zebrafish, with an emphasis on anxious behaviors. Because cannabinoids are largely non-polar substances that demonstrate higher affinity for non-polar solvents than with water, we plan to extract cannabinoids from hemp with hexane, then into DMSO for dosing the zebrafish subjects. If we can demonstrate an effect, we hope to expand into comparisons with extracts containing different proportions of cannabinoids and full-spectrum extracts of hemp flower that contain terpenes and flavonoids in addition to cannabinoids.