Extraction & Identification Of Nicotine From Hookah Tobacco Leaves Using Gas Chromatography & Reversed-Phase High- Performance Liquid Chromatography
Abstract
The spread of hookah smoking among the world’s young people necessitates further study of addiction to this type of smoking. Nicotine, a plant alkaloid found in tobacco leaves, can cause addiction. In this study, a high-performance liquid chromatographic method was developed to identify nicotine extracted from hookah tobacco leaves. The study tried several extraction methods to make a suitable sample for the HPLC instrument to be tested. However, solvent extraction using methanol was chosen to prepare the sample tested in this study. Using C8 column from Water XTerra MS, isocratic reversed phase mode with a retention time around five minutes. The High-Performance Liquid Chromatography optimum parameters were a mobile phase composition of 40% solvent B methanol: 60% solvent A phosphate buffer (40:60). Solvent A was made from 25mM potassium phosphate dibasic pH 7.5, flow rate of 1 mL/min, with 260nm absorbance value and ambient temperature value. The method was validated for stability, precision, accuracy, limit of detection, and limit of quantification. The researchers found the method results stable and linear in concentrations range between 10 ppm to 0.625 ppm with 0.999 correlation coefficient value.
Extraction & Identification Of Nicotine From Hookah Tobacco Leaves Using Gas Chromatography & Reversed-Phase High- Performance Liquid Chromatography
The spread of hookah smoking among the world’s young people necessitates further study of addiction to this type of smoking. Nicotine, a plant alkaloid found in tobacco leaves, can cause addiction. In this study, a high-performance liquid chromatographic method was developed to identify nicotine extracted from hookah tobacco leaves. The study tried several extraction methods to make a suitable sample for the HPLC instrument to be tested. However, solvent extraction using methanol was chosen to prepare the sample tested in this study. Using C8 column from Water XTerra MS, isocratic reversed phase mode with a retention time around five minutes. The High-Performance Liquid Chromatography optimum parameters were a mobile phase composition of 40% solvent B methanol: 60% solvent A phosphate buffer (40:60). Solvent A was made from 25mM potassium phosphate dibasic pH 7.5, flow rate of 1 mL/min, with 260nm absorbance value and ambient temperature value. The method was validated for stability, precision, accuracy, limit of detection, and limit of quantification. The researchers found the method results stable and linear in concentrations range between 10 ppm to 0.625 ppm with 0.999 correlation coefficient value.