Separation, Identification, and Quantification of Epothilone B using High Performance Liquid Chromatography Mass Spectrometry.
Location
SU-217
Department
Chemistry
Abstract
Secondary metabolites are chemical compounds produced by living organisms such as plants, fungi, and microorganisms through their metabolic processes. Natural products are used in a variety of industries including agriculture, beauty products, biotechnology, and medicine. In medicine, many of these naturally occurring compounds have therapeutic use, particularly as antibacterial, antifungal, and anticancer compounds. Myxobacteria, a group of soil-dwelling bacteria found in various terrestrial habitats, produce a diverse assortment of secondary metabolites. High-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) and Nuclear Magnetic Resonance (NMR) have been employed to identify and quantify more than 100 compounds formed during the growth and development of myxobacteria. This presentation will focus on the separation, identification, and quantification of Epothilone B using HPLC-MS. Epothilone B is a natural product of the myxobacterium Sorangium cellulosum and has shown promise in cancer research and treatment due to its ability to inhibit cell division and induce apoptosis in cancer cells. Some synthesized derivatives of Epothilone B, such as ixabepilone, have shown great efficacy in clinical trials. This presentation will describe the HPLC-MS method development, with Epothilone B as a standard, that will be used to analyze supernatant solutions where myxobacteria have been grown. The ultimate goal of this research is to determine if environmental factors, including the presence of light, impact the chemical identity and quantity of the produced natural products. If successful, this research project will provide new insight into the metabolic pathways of myxobacteria and may provide new target compounds to test for therapeutic use.
Faculty Sponsor
Kenneth Nicholson
Faculty Sponsor
Emina Stojkovic
Separation, Identification, and Quantification of Epothilone B using High Performance Liquid Chromatography Mass Spectrometry.
SU-217
Secondary metabolites are chemical compounds produced by living organisms such as plants, fungi, and microorganisms through their metabolic processes. Natural products are used in a variety of industries including agriculture, beauty products, biotechnology, and medicine. In medicine, many of these naturally occurring compounds have therapeutic use, particularly as antibacterial, antifungal, and anticancer compounds. Myxobacteria, a group of soil-dwelling bacteria found in various terrestrial habitats, produce a diverse assortment of secondary metabolites. High-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) and Nuclear Magnetic Resonance (NMR) have been employed to identify and quantify more than 100 compounds formed during the growth and development of myxobacteria. This presentation will focus on the separation, identification, and quantification of Epothilone B using HPLC-MS. Epothilone B is a natural product of the myxobacterium Sorangium cellulosum and has shown promise in cancer research and treatment due to its ability to inhibit cell division and induce apoptosis in cancer cells. Some synthesized derivatives of Epothilone B, such as ixabepilone, have shown great efficacy in clinical trials. This presentation will describe the HPLC-MS method development, with Epothilone B as a standard, that will be used to analyze supernatant solutions where myxobacteria have been grown. The ultimate goal of this research is to determine if environmental factors, including the presence of light, impact the chemical identity and quantity of the produced natural products. If successful, this research project will provide new insight into the metabolic pathways of myxobacteria and may provide new target compounds to test for therapeutic use.